How to avoid contamination issues in siRNA synthesis?

It is crucial to avoid contamination during the synthesis of siRNA, as contamination may seriously affect the synthesis efficiency and quality of siRNA. Here are some common methods to avoid pollution problems:

1. Strictly control experimental conditions: In siRNA synthesis experiments, it is necessary to maintain the cleanliness and tidiness of the laboratory to avoid the entry of harmful substances and microorganisms. The hygiene of the experimental environment can be maintained by regularly cleaning the laboratory table and using aseptic techniques.

2. Use high-purity reagents: siRNA synthesis experiments require the use of various reagents and substances, such as nucleotides, enzymes, etc. To avoid introducing impurities and harmful substances, high-purity reagents need to be selected and tested before use.

3. Strictly control reaction conditions: The siRNA synthesis reaction requires precise control of factors such as temperature, pH value, ion concentration, etc. to ensure stable reaction. During the operation, it is necessary to avoid being affected by the external environment and avoid introducing pollutants.

4. Pay attention to avoiding cross contamination: In siRNA synthesis experiments, it is often necessary to process multiple samples simultaneously. To avoid cross contamination between samples, specialized experimental equipment and tools need to be used, and operating procedures must be strictly followed.

5. Regular inspection and cleaning of equipment: siRNA synthesis experiments require the use of various equipment and instruments, such as PCR machines, analytical instruments, etc. To avoid pollution issues, it is necessary to regularly inspect and clean these devices and keep them in good working condition.

6. Use quality controlled carriers: In siRNA synthesis experiments, carriers are often needed to deliver siRNA. To avoid introducing impurities and harmful substances, it is necessary to choose a carrier with controllable quality and pay attention to storage and usage conditions.

7. Take personal protective measures: In siRNA synthesis experiments, some reagents and substances may have certain toxicity. To protect oneself and others' safety, it is necessary to take corresponding personal protective measures, such as wearing gloves, masks, etc.

In general, avoiding contamination issues in siRNA synthesis experiments requires comprehensive consideration of various factors such as experimental conditions, reagent quality, and operating procedures, and strict adherence to corresponding operating procedures and standard operating procedures. Only in this way can the quality and accuracy of siRNA synthesis experiments be effectively guaranteed.