Sequence Design and Screening

Sequence Design and Screening

RNAi is achieved by binding siRNA fragments to target genes and degrading them. Therefore, ensuring highly homologous siRNA sequences to target genes without homologous sequences to other genes is the key to determining the specificity of RNAi and the basic principle of siRNA design. siRNA design is based on multiple factors, including: the position of siRNA sequence in the target gene; The starting base and length of siRNA sequence; Selection of G/C content; Avoid consecutive single bases and reverse repetitive sequences; Internal stability; The base preference of the justice chain; Factors such as sequence specificity. Simultaneously selecting efficient siRNA sequences from siRNA sequences with different silencing efficiencies requires rigorous design and continuous experimental verification.

Biosyntech can design siRNA according to customer requirements, use professional design software to comprehensively design sequences with high predictive potential, and then conduct comprehensive evaluation, off target effect prediction, and professional analysis. Exposed siRNA has poor stability and low specificity, making sequence chemical modification particularly important. Biosyntech has very mature experience in chemical modification and can provide chemical modification services.

At present, Biosyntech has established a professional oligonucleotide biology evaluation platform and a complete high-throughput drug screening system, which can provide Oligonucleotide drug screening services, including high-throughput screening and optimization, confirmation of activity through re screening, professional high-throughput screening analysis reports, etc. The experimental throughput, screening efficiency, and experimental reproducibility can reach a high level.